Beeston missed out on an opportunity to close the gap on second and cement third place as they fell to a 6-4 defeat at Reading.
Slough and Beeston played out a 0-0 draw and with it, ended any hopes of a Beeston play-off appearance with Slough still in the firing line for relegation.
Beeston cemented third spot and dealt a blow to Hampstead & Westminster with a 1-0 victory at home.
Buckingham climbed to fourth in the Investec Women’s Hockey League Premier Division with a 2-1 win over Beeston, boosting their hopes of reaching the play-offs.
Third-placed East Grinstead closed the gap on second place as they won 5-0 at Beeston.